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EmCD CHO® 121 Basal Medium

Catalog No.

Product Name

Appearance

Volume

L10700.1000

EmCD CHO® 121 Basal Medium

Liquid

1L

P10700.0010

EmCD CHO® 121 Basal Medium

Powder

10L

P10700.0100

EmCD CHO® 121 Basal Medium

Powder

100L

P10700.0500

EmCD CHO® 121 Basal Medium

Powder

500L

Model:
Volume:
Availability:

Product Overview

EmCD CHO® 121 Basal Medium is precisely formulated to tackle the challenges of scaling up CHO-S cells in bioreactors, ensuring the highest quality and expression. This medium has also been shown to significantly improve protein expression in CHO-K1 cells as well in fed-batch process. This chemically defined products, devoid of protein and animal-origin components, guarantee high productivity and consistent performance, and simplify transfer to large-scale manufacturing.


Key Features

  • Achieve Higher Expressions: When paired with EmCD CHO® 121 Feed, EmCD CHO® 121 Basal Medium is fortified to help maximize CHO cell growth and protein expression in fed-batch culture, yielding up to 4.7 times higher product expression compared to just glucose-supplemented batch cultures by day 14.

  • Streamline Scalability and Accelerate Process Development: EmCD CHO® 121 Series Medium deliver the power to achieve a high cell density swiftly, outperforming commercial media in both shake flasks and bioreactors. The remarkable cell growth rates make it an ideal choice for scale-up. Alongside its compatibility with other CHO cell lines, including CHO-K1, CHO-S, and CHO-DG44, it provides a versatile and efficient way to develop processes.


Considering to Use EmCD CHO® 121 Basal Medium

  • When addressing rapid cell death, declining cell density, or low expression in the fed-batch process scale-up of CHO-S cell lines.

  • Seeking a platform medium adaptable to various CHO cell subtypes for growth and expression.


Product Information

Type:

Chemically Defined Medium

Cell Line:

CHO-S, CHO-K1, CHOZN, CHO-DG44

Appearance:Liquid/Powder
Packaging:Bottle/Barrel
Specification:1L, 10L, 100L, 500L


Components

Animal-component free, Protein free, Growth-factors free, Glutamine free


Data Chart

图片1
图片2
图片3

Utilizing the EmCD CHO® 121 Basal Medium in conjunction with the EmCD CHO® 121 Feed for fed-batch cultures of CHO-S cell lines leads to a significant enhancement in cell conditions compared to batch cultures that solely add glucose. The highest live cell density reaches 25×10^6 cells/mL, with a viability rate exceeding 90% on Day 14, and a product expression that increases by 4.7 times


图片4 Utilizing the EmCD CHO® 121 Basal Medium in conjunction with the EmCD CHO® 121 Feed for fed-batch cultures of CHO-K1 cell lines has better growth curve to that of commercialized media
图片5 The cell viability maintained in fed-batch culture process with the EmCD CHO® 121 series medium is superior to that of commercialized media, with the viability in Day 14 higher than 90%
图片6

The Titer of EmCD CHO® 121 series culture medium in fed-batch culture process of CHO-K1 cells is 19% higher than the leading international media, reaching 6.9g/L


Storage & Logistic

Storage Conditions: 2°C to 8°C, protect from light

Shipping Conditions: Ambient

Expiration Date of Liquid Product: 12 Months

Expiration Date of Powder Product: 24 Months


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